Spectrométrie de Masse Biologique et Protéomique, SMBP CNRS USR3149

Edito

The unit’s main project is the elaboration of specific, sensitive and performing methodologies for the identification and the characterization of proteins, peptides and other peptidomimetic molecules such as toxins. Protein analysis in proteomic studies generally combine two-dimensional electrophoresis to MALDI-TOF MS detection (Matrix Assisted Laser Desorption Ionisation–Time Of Flight) to achieve peptide mapping. The MALDI ionization mode can be employed in tissue imaging by localizing specific molecules such as peptides and proteins directly from tissue slices or cells, both healthy and pathological, in order to demonstrate the presence of certain bio-markers for example. Moreover, the emergence of liquid nano-chromatographic techniques in both one (1D-LC) and two-dimensional (2D-LC) configurations coupled to tandem mass spectrometers (MS/MS), such as hybrid instruments like QqTOF (Quadripole-Time Of Flight) or trap LIT-FTICR (Linear Ion Trap-Fourier Transform Ion Cyclotron Resonance) are an attractive approach by their capacity to fragment peptides that are present in very small amounts, in order to obtain amino acid sequence information. This coupling allows the skipping of the two-dimensional electrophoresis separation step and thus the characterization of all the proteins as well as their post-translational modifications directly from the mixture. MALDI ionization can also be coupled to MS/MS analysis in order to generate sequence informations directly from preparations having served to establish peptide maps for example.

Note
TGE FTICR is changing and has been converted in the CNRS FR3624! see here =>
Job opportunities! see here =>



Plateform of mass spectrometry and proteomics(english)

3 Installation of autosampler Nanomate triversa!

The LTQ-FT ultra is now fully equipped with an autosampler Nanomate Advion that eases the (...)


> All featured stories...

News

3 Installation of autosampler Nanomate triversa!

The LTQ-FT ultra is now fully equipped with an autosampler Nanomate Advion that eases the (...)


Mass Spectrometry and Proteomics training : Monday, December 1st - Friday, December 5th 2014

GOALS Acquiring the theoretical bases and minimum practical requirements to interpret mass (...)


> More news...

Publications

Publications 2011-2013


2013

Tau pathology modulates Pin1 post-translational modifications and may be relevant as...

(...)

> More publications...

Top